DNA quality

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H “DNA Quality Requirements for Next Generation Sequencing (NGS) – MR DNA”, tailored for MR DNA, MR DNA is also specialists in using difficult DNA so just contact us at info@mrdnalab.com for all you next generation sequencing needs

DNA Quality Requirements for Next Generation Sequencing (NGS)

Ensure Accurate Results with High-Quality DNA – MR DNA

At MR DNA, we are committed to delivering precise, high-throughput results through cutting-edge next generation sequencing (NGS) services. One of the most important factors affecting sequencing accuracy, coverage, and interpretability is the quality and integrity of the input DNA.

Whether you’re submitting DNA for shotgun metagenomics, amplicon sequencing, whole genome sequencing, or virome analysis, adhering to our recommended DNA quality guidelines ensures the best possible outcomes.

📌 Typical DNA Quality and Quantity Requirements

To maximize sequencing efficiency and data quality, please follow the DNA submission guidelines below:

✔️ Concentration and Volume

  • Minimum concentration: 5–20 ng/µL (project-dependent)
  • Preferred total input: ≥100 ng of DNA
  • Volume: At least 20 µL (in nuclease-free water or low-EDTA TE buffer)

✔️ Purity (Spectrophotometer Ratios)

  • A260/A280: 1.8–2.0 (indicates minimal protein contamination)
  • A260/A230: >1.8 (indicates minimal salt/solvent contamination)

✔️ Integrity

  • DNA should be high molecular weight and intact
  • Avoid shearing or degradation (verify with gel electrophoresis or TapeStation/Fragment Analyzer)
  • No visible smearing or <500 bp fragments

🚫 Common Reasons for Sample problems

  • Presence of PCR inhibitors (e.g., humic acid, phenol, ethanol)
  • Low concentration or insufficient volume
  • Degraded or sheared DNA
  • Contamination from RNA, proteins, or organic solvents

🧬 Sample Types Accepted (any)

We work with a variety of sample sources including:

  • Environmental DNA (soil, water, sediment)
  • Microbiome (fecal, oral, vaginal, skin)
  • Tissue and biopsy samples
  • Clinical isolates
  • Viral, fungal, and bacterial cultures

Each sample type may have specific handling and extraction considerations—please contact us if you’re unsure.

🧪 Tips for High-Quality DNA Extraction

To improve DNA quality:

  • Use validated DNA extraction kits with inhibitor removal columns (e.g., QIAGEN, Zymo)
  • Avoid vortexing or excessive pipetting
  • Elute in nuclease-free water or low-EDTA buffer
  • Store at -20°C and avoid freeze-thaw cycles

🔍 Why DNA Quality Matters for NGS

High-quality DNA is essential for:

  • Accurate taxonomic assignment in metagenomics
  • Efficient library preparation
  • Uniform genome coverage
  • Reduced sequencing bias and failure rates

Poor DNA quality can result in:

  • Low read counts
  • Incomplete or inaccurate assemblies
  • Elevated error rates and discarded reads

💬 Questions? Contact Our NGS Specialists

Have questions about your DNA prep or need extraction services? Our team at MR DNA is ready to assist with your sequencing project from start to finish.

📧 Email: info@mrdnalab.com
📞 Phone: 1-866-573-2710
🌐 Learn more: www.mrdnalab.com

🧡 Trusted by Researchers Worldwide

MR DNA is a pioneer in microbiome and metagenomic sequencing, serving thousands of academic, clinical, and environmental researchers across the globe.

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